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The arrangement of cells within a tissue plays an essential role in organogenesis, including tooth development. Three protocols were compared. Progress is being made to regenerate teeth by reassociating dissociated embryonic dental cells and implanting them in vivo. In the present study, we tested the hanging drop method to study mixed epithelial-mesenchymal cell reorganization in a liquid instead of semisolid medium to see whether it could lead to tooth histogenesis and organogenesis. Our investigation allowed us to build a detailed map of tumor vasculature in human Kaposi sarcoma. The latest therapies aim to limit its intrinsic angiogenic activity in an attempt to reduce vascular density and the formation of new vessels. This experimental model can be employed to study the development, physiology, metabolism, or toxicology in forming teeth and test other cell sources.

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In the present study, we tested the hanging drop method to study mixed epithelial-mesenchymal cell reorganization in a liquid instead of semisolid medium to see whether it could lead to tooth histogenesis and organogenesis.

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Three-dimensional Micro-culture System for Tooth Tissue Engineering.

We used a corrosion casting technique applied to nude mice previously transplanted subcutaneously with human modified neoplastic Kaposi sarcoma cells. In the present study, we tested the hanging drop method to study mixed epithelial-mesenchymal cell reorganization in a liquid instead of semisolid medium to see whether it could lead to tooth histogenesis and organogenesis. Vascularization and the cellular heterogeneity in the mesenchyme were similar to what was observed in developing molars. Three protocols were compared. This method allowed the control of the proportion and number of cells to be used, and the forming microtissues showed homogeneous size. Its clinical presentation and anatomopathological structure seem to demonstrate a particularly rich vascularity. This experimental model can be employed to study the development, physiology, metabolism, or toxicology in forming teeth and test other cell sources.

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